Dionex ProPac IMAC Column 生物色譜柱

簡(jiǎn)要描述：

戴安公司生產(chǎn)的儀器主要有離子色譜儀、高效液相色譜儀、快速溶劑萃取儀和氨基酸直接分析儀。其中離子色譜儀憑借著雄厚的技術(shù)優(yōu)勢(shì)和方法已廣泛地應(yīng)用于各個(gè)領(lǐng)域，其*達(dá)80%。與離子色譜相關(guān)的文章有90%均出自戴安公司生產(chǎn)的離子色譜儀。　　
戴安公司的快速溶劑萃取儀15分鐘之內(nèi)僅用15毫升溶劑既可完成對(duì)10g樣品的萃取，該技術(shù)已在環(huán)境、藥物、食品和聚合物工業(yè)等領(lǐng)域得到廣泛應(yīng)用。

型號(hào)	美國(guó)戴安 063276
廠商性質(zhì)	代理商
更新日期	2018-04-15

詳細(xì)介紹

Analytical Column for Immobilized Metal Affinity Chromatography

The ProPac^? IMAC-10 is an analytical HPLC column packed with 10 μm, nonporous, polymeric beads that are coated with a hydrophilic layer, then grafted with poly(IDA) chains. The poly(IDA) grafts are converted to metal-containing nanoparticles when the column is charged with metal. It is these nanoparticles that act as IMAC interaction sites for individual proteins and provide the ProPac IMAC-10 with its high resolving power.

State-of-the-art technology for reusable columns with metal tailored specificity
Resolve target proteins using a single column in a high resolution gradient run
Retention control by imidazole concentration or pH gradient
High purity separations of metal-binding proteins
Wide range of metal-specific applications

The ProPac IMAC-10 design maximizes the number of sterically accessible ligands for binding to proteins, ensuring that the proteins remain tightly bound during separation. It can be operated between pH 2–12 and is compatible with commonly used protein purification reagents (non-ionic detergents, reducing agents). It can be used under native or denaturing HPLC conditions

The ProPac IMAC-10 is shipped free from any metal and ready to be charged with the metal of your choice. To avoid contact of the metal solution with your system, charge the IMAC-10 with a loading column (an empty column body you fill with your metal solution, then place between the pump and the IMAC-10 column).

ProPac IMAC Columns Specifications
Maximum Operating Pressure	<3000 psi (21 MPa)
Temperature Range	<60^oC Recommended Temperature: Controlled ambient
Typical Operating Conditions	<600 psi at 1.0 mL/min (4 × 250mm) Recommended flow rate: 1.0 mL/min
Phase Compatibility	pH 2–12, Compatible with most reagents used in IMAC protein purifications, including EtOH, Urea, NaCl, non-ionic detergents, glycerol, acetic acid, guanidine, HCl
Resin Compostion	10 μm diameter nonporous polystyrene divinylbenzene substrate (55% crosslinking) with poly(IDA) grafts.
Metal Ion Capacity (μmol Cu)	>80 mmol Cu (4 × 250 mm analytical column)
Chelating Metals	Ni, Fe, Cu, others
Analytical Column Dimensions	1 × 50 mm, 2 × 50 mm, 4 × 50 mm, 9 × 50 mm 2 × 250 mm, 4 × 250 mm, 9 × 250 mm, 22 × 250 mm
Loading Column Dimensions	4 × 50 mm, 9 × 50 mm, 9 × 250 mm

Analytical Columns
ProPac IMAC-10 Column (1 x 50 mm)	063617
ProPac IMAC-10 Column (2 x 50 mm)	063272
ProPac IMAC-10 Column (4 x 50 mm)	063276
ProPac IMAC-10 Column (9 x 50 mm)	063615
ProPac IMAC-10 Column (2 x 250 mm)	063274
ProPac IMAC-10 Column (4 x 250 mm)	063278
ProPac IMAC-10 Column (9 x 250 mm)	063280
ProPac IMAC-10 Column (22 x 250 mm)	063282

Accessories
IMAC Loading Column (4 x 50 mm)	063667
IMAC Loading Column (9 x 50 mm)	063710
IMAC Loading Column (9 x 250 mm)	063718

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Dionex ProPac IMAC Column 生物色譜柱

簡(jiǎn)要描述：

Analytical Column for Immobilized Metal Affinity Chromatography

留言框

產(chǎn)品：

您的單位：

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聯(lián)系電話：

常用郵箱：

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