for High-Resolution Separation of Proteins and MAbs
New! The Thermo Scientific ProPac? SCX column line has expanded to include the next generation ProPac SCX-20 column. This column provides unequalled high resolution of protein variants.
Please also see the Thermo Scientific MAbPac? SCX-10 column, a strong cation-exchange column designed specifically for high-resolution, high-efficiency analysis of monoclonal antibodies (MAbs) and associated variants. This column is complimentary to the industry-leading ProPac WCX-10 column for monoclonal antibody variant analysis. The MAbPac SCX-10 offers an alternative selectivity and provides higher resolution and efficiency for variant analysis of most monoclonal antibody samples than the ProPac WCX-10 column.
ProPac SCX-10 and WCX-10 strong and weak cation-exchange columns are based on a nonporous core particle that results in high-efficiency peaks and high-resolution separations of protein variants. A hydrophilic layer prevents unwanted secondary interactions. A grafted cation-exchange surface provides pH-based selectivity control and fast mass transfer for high-efficiency separation and moderate capacity.
Characterization and quality control assessment of monoclonal antibodies and other proteins
Unequalled resolution
High-resolution separations with excellent sample recovery
Unmatched column-to-column and lot-to-lot reproducibility
Useful for characterization of related protein variants (e.g., deamidation and MAb lysine truncation variants)
Highly-efficient peaks with excellent sample recovery
Available in different dimensions and formats for flexibility in method development
The ProPac SCX-10 is a strong cation-exchange column with sulfonate functional groups, and the ProPac WCX-10 column has a carboxylate functional group. Both are based on 10 ?m nonporous, polymeric beads and coated with a proprietary hydrophilic layer.
All ProPac columns are manufactured and tested under the strictest specifications, resulting in unmatched column-to-column and lot-to-lot reproducibility. These columns are available as lot-select packages containing three columns from a single lot or three columns from three different lots, to assist quality control scientists in method validation.
ProPac SCX-10 Strong Cation-Exchange Column for the High-Resolution Separation of Protein Isoforms Specifications
Maximum operating pressure
3000 psi (21 MPa)
Maximum operating temperature
60 °C Recommended operating temperature: <40 °C
Typical operating conditions
UV detection at 1 mL/min Recommended flow rate: 1 mL/min
phase compatibility
pH 2–12; 80% compatible with acetonitrile, acetone, methanol. Compatible with nonionic and zwitterionic detergents. Incompatible with cationic detergents.
Resin composition
10-?m-diameter core bead (55% cross-linked) grafted with a pellicular layer to which are attached the sulfonate functional groups.
Anion-exchange capacity
< 100 ?g protein on-column, depending upon the protein (4 × 250 mm analytical column)
Column construction
PEEK with 10-32 threaded ferrule-style end fittings. All components are nonmetallic.
ProPac WCX-10 Weak Cation-Exchange Column for the High-Resolution Separation of Protein Isoforms Specifications
Resin composition
10-?m-diameter core bead (55% cross-linked) grafted with a pellicular layer to which are attached the carboxylate functional groups.
Anion-exchange capacity
< 100 ?g protein on-column, depending upon the protein (4 × 250 mm analytical column)
Column construction
PEEK with 10-32 threaded ferrule-style end fittings. All components are nonmetallic.
Maximum operating pressure
3000 psi (21 MPa)
Maximum operating temperature
60 °C Recommended operating temperature: <40 °C
Typical operating conditions
UV detection at 1 mL/min Recommended flow rate: 1 mL/min
phase compatibility
pH 2–12; 80% compatible with acetonitrile and acetone. Compatible with nonionic and zwitterionic detergents. Incompatible with cationic detergents.
SCX Analytical Columns
ProPac SCX-20 Analytical Column (4 x 250 mm)
074628
ProPac SCX-10 Analytical Column (4 x 250 mm)
054995
ProPac SCX-10 Analytical Column (2 x 250 mm)
063456
ProPac SCX-10 Semi-Preparative Column (9 x 250 mm)
063700
ProPac SCX-10 Semi-Preparative Column (22 x 250 mm)
SP5522
ProPac SCX-10 Lot Select Column Set (4 x 250 mm) (1 Lot of Resin)
SP5727
ProPac SCX-10 Lot Select Column Set (4 x 250 mm) (3 Lots of Resin)
SP5728
SCX Guard Columns
ProPac SCX-20 Guard Column (4 x 50mm)
074643
ProPac SCX-10G Guard Column (4 x 50 mm)
079930
ProPac SCX-10G Guard Column (2 x 50 mm)
063462
WCX Analytical Columns
ProPac WCX-10HT Analytical Column (4x 50mm)
074600
ProPac WCX-10 Analytical Column (4 x 100 mm)
SP5829
ProPac WCX-10 Analytical Column (4 x 150 mm)
SP6703
ProPac WCX-10 Analytical Column (4 x 250 mm)
054993
ProPac WCX-10 Lot Select 3 Column Set (4 x 250 mm) (1 Lot of Resin)
SP5512
ProPac WCX-10 Lot Select 3 Column Set (4 x 250 mm) (3 Lots of Resin)
SP5513
ProPac WCX-10 Analytical Column (2 x 250 mm)
063472
ProPac WCX-10 Semi-Preparative Column (9 x 250 mm)
063474
ProPac WCX-10 Semi-Preparative Column (22 x 250 mm)
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