The Thermo Scientific Acclaim HILIC-10 column is designed for separating highly hydrophilic molecules by hydrophilic interaction liquid chromatography (HILIC). This column is suited for use in a broad range of applications, including separation of hydrophilic drugs and drug metabolites.
Retains highly polar molecules that are not retained by reversed-phase chromatography
Unique selectivity, complementary to reversed-phase columns
Hydrolytically stable
Rugged column packing
Broad application range
The advantage of the Acclaim HILIC-10 phase is the compatibility with up to 20% aqueous phase, while maintaining affinity for polar analytes. Acclaim HILIC-10 columns are available in 3 μm particle size. A variety of column formats are available: 4.6 × 150 mm, for high-throughput routine analysis; 3.0 × 150 mm, for high-throughput analysis with reduced solvent consumption; and 2.1 × 150 mm, for LC-MS analysis.
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Acclaim HILIC-10 Column Specifications
Column chemistry
Proprietary polar group
Operating pressure limit
8500 psi
Operating pH range
2.0–8.0
Operating flow rate range (recommended)
0.5–3.0 (0.8–1.6) mL/min for 4.6 mm i.d. formats 0.3–1.5 (0.4–0.8) mL/min for 3.0 mm i.d. formats 0.15–0.80 (0.2–0.4) mL/min for 2.1 mm i.d. formats
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